question and answer

What is the tip of pipette?

Pipette Tips are disposable, autoclavable attachments for the uptake and dispensing of liquids using a pipette. Micropipettes are used in a number of laboratories. A research/diagnostic lab can use pipette tips to dispense liquids into a well plate for PCR assays.

 

How many times can you use a pipette tip?

One box of pipette tips might look like a lot, but consider this: A pipette tip can only be used once. After you have used the pipette tip, it is contaminated by liquid you are pipetting. Usually you dispose of that tip and use a new pipette tip for the next sample.

 

Who uses pipette tips?

Filtered pipette tips help to reduce the risk of aerosols forming in the laboratory. They also protect the pipette shafts from contamination and reduce the risk of cross contamination. These pipette tips are often used in contamination sensitive applications such as forensics and clinical diagnostics.

 

Are all pipette tips the same size?
Universal tips are designed to fit securely and tightly around all pipette barrels, which vary slightly in diameter from manufacturer to manufacturer. But of course, all universal tips are not made equal, so you must carefully examine the choices.

 

How are pipette tips used?

Shaped like cones, a scientist attaches a pipette tip to the end of a pipettor and then uses it to transfer the liquid. The liquid used in the pipette tip is measured in microliters (μL), or one-thousandth of a milliliter (mL). Pipette tips are important materials to have in your lab for many reasons.

 

When should you use filtered pipette tips?

Sterile Filter Tips: A filter tip is beneficial when the assay is sensitive to cross-contamination or the sample can contaminate the lower part of the pipette. The filter prevents liquid from splashing accidentally inside the pipette and aerosols from penetrating into the pipette tip cone during pipetting.

 

Do you need sterile tips for PCR?

Always Use Sterile Tips for Setting up PCR Reactions
Using filter tips is recommended for these sensitive reactions but not mandatory. Filter tips have aerosol barriers which prevent contamination from air and within the tip while pipetting.

 

Why are there filter in pipette tips?

Filter tips are used to prevent cross contamination or “carry over” from one sample to another. When samples are aspirated into a pipette tip, a fine mist or “aerosol” is created, which can get into the pipette.

 

What is a universal pipette tip?

Nest Pipette Tips are ultra-clear and universal fit to most pipettes. Made from 100% polypropylene, low-retention plastic, these tips ensure accurate sample results during liquid handling. The Nest Pipette Tip line is packaged Rnase/Dnase, pyrogen free.

 

What plastic are pipette tips made of?

Pipette tips, micro test tubes, deepwell plates, microplates and Combitip cylinders are made of PP (polypropylene). PP is a relatively inert plastic characterized by low wettability as well as high dimensional and mechanical stability across a wide temperature range.

 

Do I need filter tips?

Filter tips should be used whenever you need to prevent contamination. So specifically for anything to do with RNA work, quantitative PCR and cell culture (eg small amounts of drug treatments).

 

Why is it important to use aerosol filter tips when setting up a PCR reaction?

Filter tips are vitally important for preventing contamination during PCR experiments and other molecular biology applications such as bacteriology and work in radioactive areas. Primarily, they prevent the sample solution from contaminating the pipette cone during aspiration.

 

What are sterile pipette tips?
using these racked, sterile filter pipette tips. These universal tips are manufactured from polypropylene and the filters are manufactured from polyethylene with a hydrophobic design to prevent liquids and aerosols from entering the body of the micropipette.

 

What are pre sterile tips?

It might say 'pre-sterile' on the shipping label, but this only means that the product was sterile leaving the manufacturer. The manufacturer has no responsibility after that. Using 'pre sterile' tips carries with it the risk of contaminating experiments involving an automated liquid handling system.

 

Do sterile pipette tips expire?

All VistaLab Technologies sterile disposables (Tips, Reagent Reservoirs, Wobble-not serological pipets) are good for 3 years from the Date of Manufacture found on the Certificate of Analysis included in each case; as long as the packaging is unopened and uncompromised. Non-sterile tips do not expire.

 

Why are only sterile pipette tips used in lab?

Why are only sterile pipette tips used in the lab? ... To avoid contamination of the sample. You just studied 4 terms!

 

What does low retention mean for pipette tips?

Low retention tips, also called low binding tips, are pipette tips that have been modified to reduce the adhesion of DNA, enzymes, proteins, cells, as well as other viscous materials to the tip surface.

 

Why is a well fitting tip essential when using an automatic pipette?

The connection between tip and pipette cone needs to be tight enough to prevent air passing through. If the tip fit is not tight, the system does not aspirate enough liquid and may leak.

 

What is the main purpose of disposable tips on a micropipette?

Instead, a disposable pipette tip is used to draw the liquid into and dispense from the micropipette. So in order to allow the safe, effortless and quick ejection of tips, micropipettes are provided with a tip ejection system. The tips can be easily removed from the micropipette by pressing the tip ejector button.

 

What is the difference between filtered and unfiltered pipette tips?

The most commonly used type of pipette tip is non-sterile tips. They are often used in laboratory applications where sterility is not important to the experiment or test being performed. ... Filtered pipette tips are designed to prevent aerosols from forming. Aerosols are small liquid or solid particles that are airborne.

 

What do you need filter tips for?

Filter tips are generally accepted as the solution for preventing cross-contamination of samples during pipetting tasks in applications such as bacteriological work, PCR and radioimmunoassays.

 

How do filtered pipette tips work?

Aerosol Barrier tips, also called filter pipette tips, are fitted with a filter inside the proximal part of the tip. The filter protects your pipettes from aerosols and aspirating volatile or viscous solutions into the barrel, all of which can contaminate and damage the pipette.

 

What is the volume range of P1000?

P1000: Maximum volume 1000 µl (= 1 ml). Accurate between 200 µl and 1000 µl.

 

Do you need filtered tips for PCR?

Sterilized Filter Tip use is indispensable to prevent aerosol contamination when using PCR and amplification methods, pipetting RNA/DNA solutions, infectious samples, etc.